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You are here: Home staff publications Publications Hans-Jürgen Ensikat

Publications Hans-Jürgen Ensikat

SEM Preparation Methods

Ensikat, H. J.; Weigend, M. (2014): SEM of Biological Samples without Coating – Utilizing Bulk Conductivity for Increased Versatility. Imaging and Microscopy, 2014, 16: 34-36.

Ensikat, H. J.; Weigend, M. (2013): Cryo-scanning electron microscopy of plant samples without metal coating, utilizing bulk conductivity. Microscopy and Analysis, 2013, 27(6): 7-10.

Ensikat, H. J. (2012): SEM-based diagnostic tools to determine the quality of superhydrophobic surfaces. In: Zinigrad, M.; Lugovskoy, A., (Eds.); Proceedings of the 7th International Conference MMT-2012. Ariel University Center of Samaria, Ariel, Israel.

Ensikat, H. J.; Mayser, M.; Barthlott, W. (2012): Superhydrophobic and adhesive properties of surfaces: Testing the quality by an elaborated scanning electron microscopy method. Langmuir, 2012, 28(40): 14338–14346

Ensikat, H.J., Ditsche-Kuru, P., Barthlott, W. (2010): Scanning electron microscopy of plant surfaces: simple but sophisticated methods for preparation and examination. In: Méndez-Vilas, A.; Alvarez, J. D., (Eds.); Microscopy: Science, Technology, Applications and Education. Vol. 1 (13); Formatex Research Center, Bajados, Spain; pp 248-255.

Ensikat, H.J., Schulte, A.J., Koch, K., Barthlott, W. (2009): Droplets on Superhydrophobic Surfaces: Visualization of the Contact Area by Cryo-Scanning Electron Microscopy. Langmuir, 2009, 25(22): 13077-13083. DOI: 10.1021/la9017536

Ensikat, H.J., Neinhuis, C., Barthlott, W. (2000): Direct access to plant epicuticular wax crystals by a new mechanical isolation method. Int. J. Plant Sci. 161(1): 143-148.

Šamaj, J., Ensikat, H.J., Baluska, F., Knox, J.P., Barthlott, W., Volkmann, D. (1999): Immunogold localization of plant surface arabinogalactan-proteins using glycerol liquid substitution and scanning electron microscopy. J. Microsc. 193: 150-157.

Ensikat, H.J. (1996): Liquid Substitution Methods. In: Procedures in Electron Microscopy. Principal Editors A.W. Robards, A.J. Wilson. JOHN WILEY & SONS. York, UK.

Ensikat, H.J., Barthlott, W. (1993): Liquid substitution: A versatile procedure for SEM specimen preparation of biological materials without drying or coating. J. Microsc. 172: 195-203.

 

Plant Biomineralization, Trichomes, Loasaceae

Mustafa, A.; Ensikat, H. J.; Weigend, M. (2018): Stinging hair morphology and wall biomineralization across five plant families: conserved morphology versus divergent cell wall composition. American Journal of Botany, 2018, in print

Weigend, M.; Mustafa, A.; Ensikat, H. J. (2018): Calcium phosphate in plant trichomes: the overlooked biomineral. Planta, 2018, 247: 277-285.

Mustafa, A.; Ensikat, H. J.; Weigend, M. (2018): Mineralized trichomes in Boraginales: complex microscale heterogeneity and simple phylogenetic patterns. Annals of Botany, 2018, 121(4): 741-751.

Mustafa, A.; Ensikat, H. J.; Weigend, M. (2017): Ontogeny and the process of biomineralization in the trichomes of Loasaceae. American Journal of Botany, 2017, 104(3): 1-12.

Ensikat, H. J.; Mustafa, A.; Weigend, M. (2017): Complex patterns of multiple biomineralization in single-celled plant trichomes of the Loasaceae. American Journal of Botany, 2017, 104(2): 195-206.

Ensikat, H. J.; Geisler, T.; Weigend, M. (2016): A first report of hydroxylated apatite as structural biomineral in Loasaceae – plants’ teeth against herbivores. Sci. Rep. 6, 26073; doi: 10.1038/srep26073 (2016).

Hartl, W.P., Klapper, H., Barbier, B., Ensikat,H.J., Dronskowski,R., Müller, P., Ostendorp, G., Tye, A., Bauer, R., Barthlott, W. (2007): Diversity of calcium oxalate crystals in Cactaceae. Can. J. Bot. 85 (5): 501-517.

 

Epicuticular Wax, Lotus Effect, Superhydrophobicity

Ensikat, H. J. (2012): SEM-based diagnostic tools to determine the quality of superhydrophobic surfaces. In: Zinigrad, M.; Lugovskoy, A., (Eds.); Proceedings of the 7th International Conference MMT-2012. Ariel University Center of Samaria, Ariel, Israel.

Ensikat, H. J.; Mayser, M.; Barthlott, W. (2012): Superhydrophobic and adhesive properties of surfaces: Testing the quality by an elaborated scanning electron microscopy method. Langmuir, 2012, 28 (40): 14338–14346

Ensikat, H. J.; Ditsche-Kuru, P.;  Neinhuis, C.;  Barthlott, W. (2011): Superhydrophobicity in perfection: the outstanding properties of the Lotus leaf. Beilstein J. Nanotechnol. 2011, 2: 152-161

Ensikat, H.J., Schulte, A.J., Koch, K., Barthlott, W. (2009): Droplets on Superhydrophobic Surfaces: Visualization of the Contact Area by Cryo-Scanning Electron Microscopy. Langmuir, 2009, 25(22): 13077-13083. DOI: 10.1021/la9017536

Koch, K., Ensikat, H.J. (2008): The hydrophobic coatings of plant surfaces: epicuticular wax crystals and their morphologies, crystallinity and molecular self-assembly. Micron, 39: 759-772. DOI:10.1016/j.micron.2007.11.010

Mockenhaupt, B., Ensikat, H.J., Spaeth, M., Barthlott, W. (2008): Superhydrophobicity of Biological and Technical Surfaces under Moisture Condensation: Stability in Relation of Surface Structure. Langmuir, 24: 13591-13597.

Ensikat, H.J., Boese, M., Mader, W., Barthlott, W., Koch, K. (2006): Crystallinity of plant epicuticular waxes: Electron and X-ray diffraction studies. Chem. Phys. Lipids, 144: 45-59.

Koch, K., Neinhuis, C., Ensikat, H.J., Barthlott, W. (2004): Self assembly of epicuticular waxes on plant surfaces investigated by Atomic Force Microscopy (AFM). J. Exp. Bot., 55: 711-718.

Ensikat, H.J., Neinhuis, C., Barthlott, W. (2000): Direct access to plant epicuticular wax crystals by a new mechanical isolation method. Int. J. Plant Sci. 161(1): 143-148.

 

Miscellaneous, with SEM Contributions

Dehling, J. M.; Hinkel, H. H.; Ensikat, H. J.; Babilon, K.; Fischer, E. (2018): A new blind snake of the genus Letheobia (Serpentes: Typhlopidae) from Rwanda with redescriptions of L. gracilis (Sternfeld, 1910) and L. graueri (Sternfeld, 1912) and the introduction of a non-invasive preparation procedure for scanning electron microscopy in zoology. Zootaxa, 2018, 4378(4): 480-490.

Thomas, A.; Biswas, A.; Ensikat, H.J.; et al. (Institute of Experimental Haematology ..., Bonn) 2016: Coagulation Factor XIIIA Subunit Missense Mutations Affect Structure and Function at the Various Steps of Factor XIII Action. Human Mutation, 2016, 37(10): 1030-1041.

Hammouda, S. A.; Weigend, M.; Mebrouk, F.; Chacon, J.; Bensalah, M.; Ensikat, H. J.; Adaci, M. (2015): Fossil nutlets of Boraginaceae from the continental Eocene of Hamada of Méridja (southwestern Algeria): The first fossil of the Borage family in Africa. American Journal of Botany, 2015, 102: 2108-2115.

Šamaj, J., Ensikat, H.J., Barthlott, W., Volkmann, D. (2002): Immunogold-silver scanning electron microscopy using glycerol liquid substitution. In: Gu, J. & G. W. Hacker (eds.) Gold and silver staining: techniques in molecular morphology. CRC Press, Boca Raton London New York Washington D. C., pp. 223-234.

Šamaj, J., Ensikat, H.J., Baluska, F., Knox, J.P., Barthlott, W., Volkmann, D. (1999): Immunogold localization of plant surface arabinogalactan-proteins using glycerol liquid substitution and scanning electron microscopy. J. Microsc. 193: 150-157.

Reichelt, S., Ensikat, H.J., Barthlott, W., Volkmann, D. (1995): Visualization of immunogold-labeled cytoskeletal proteins by scanning electron microscopy. Euro. J. Cell Biol. 67: 89-93.

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